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KMID : 0191119980130040377
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Journal of Korean Medical Science
1998 Volume.13 No. 4 p.377 ~ p.382
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Localization of hepatitis B virus DNA in hepatocellular carcinoma by polymerase chain reaction in situ hybridization
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Shin YJ
Cho SW/Hahm KB/Kim YS/Kim JH/Park KH/Lee SI
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Abstract
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The polymerase chain reaction in situ hybridization (PCR-ISH) is a new technique that combines the
sensitivity of PCR with the localizing ability of ISH. To investigate the expression pattern of hepatitis
B virus (HBV) in the tissue of hepatocellular carcinoma (HCC), we detected HBV-DNA with PCR-ISH in
paraffin-embedded tumor and corresponding non-tumor tissues from 11 HCC patients. HBV-DNA was detected
in 4 of 11 tumor tissues and in 7 of 10 non-tumor tissues. In tumor tissues, positive signals were
scattered in the tissue with occasional clustering, and were found mainly in the cytoplasm of HCC
cells rather than in the nucleus. In non-tumor tissues, the number of positive signals was higher
than in tumor tissues and they were found in regenerating nodules with differing patterns and intensities.
When we compared the detection rate of PCR-ISH with nested PCR among 10 tissue samples, HBV-DNA was
detected in 5 tissue samples by PCR-ISH, but the S gene was detected in 10, precore gene in 9 and X
gene in 8 by nested PCR. The findings suggest that PCR-ISH is a sensitive technique for localizing HBV
in tissue sections and that the low level of HBV replication persists in HCC cells.
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KEYWORD
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